FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_IMMT_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_IMMT_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26541
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA2290886.31174409404318No Hit
CTGTTGCTGAAGCTGGAGGGAAAGATTTTTAGTGGACCCGACTGAGATCG1650.6216796654233073No Hit
CTGTTGCTGAAGCTGGATTTTTAGTGGACCCGACTGAGATCGGAAGAGCG1130.425756376926265No Hit
CTGTTGCTGAAGCTGGAGATCGGAAGATTTTTAGTGGACCCGACTGAGAT970.3654722881579443No Hit
CTGTTGCTGAAGCTGGAAGAGCGTCGTGTAGGGAAAGATTTTTAGTGGAC820.30895595493764366Illumina Single End PCR Primer 1 (96% over 26bp)
CTGTTGCTGAAGCTGGAGGGAAAGAGATTTTTAGTGGACCCGACTGAGAT800.30142044384160355No Hit
CTGTTGCTGAAGCTGGAGATCGGATTTTTAGTGGACCCGACTGAGATCGG780.29388493274556343No Hit
CTGTTGCTGAAGCTGGAGATCGGAAGAGATTTTTAGTGGACCCGACTGAG690.2599751328133831No Hit
CTGTTGCTGAAGTGGAGGGAAAGATTTTTAGTGGACCCGACTGAGATCGG520.19592328849704232No Hit
CTGTTGCTGAAGCTGGAGATCGGAAGAGCACACGATTTTTAGTGGACCCG500.18838777740100224No Hit
CTGTTGCTGAAGCTGGAAGAGCGTCGTGTAGGGAAAGAGATTTTTAGTGG470.1770845107569421Illumina Single End PCR Primer 1 (96% over 25bp)
CTGTTGCTGAAGCTGGAGATCGGAATTTTTAGTGGACCCGACTGAGATCG410.15447797746882183No Hit
CTGTTGCTGAAGTGGAGGGAAAGAGATTTTTAGTGGACCCGACTGAGATC340.1281036886326815No Hit
CTGTTGCTGAAGCTGGAGAGATTTTTAGTGGACCCGACTGAGATCGGAAG330.12433593308466147No Hit
CTGTTGCTGAAGCTGGAAAGATTTTTAGTGGACCCGACTGAGATCGGAAG320.12056817753664142No Hit
CTGTTGCTGAAGCTGGAGATCGGAAGAGGATTTTTAGTGGACCCGACTGA300.11303266644060134No Hit
CTGTTGCTGAAGCTGGAGATCGGAAGAGCACACGGATTTTTAGTGGACCC300.11303266644060134No Hit
CTGTTGCTGAAGCTGGAGGGAAAGAATTTTTAGTGGACCCGACTGAGATC300.11303266644060134No Hit
CTGTTGCTGAAGCTGGAGATTTTTAGTGGACCCGACTGAGATCGGAAGAG300.11303266644060134No Hit
TGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG270.10172939979654119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTGG152.1971202E-4125.000019
CTGAAGT152.1971202E-4125.000017
TGAAGTG152.1971202E-4125.000018
CTTTGCT152.1971202E-4125.000011
CTGTTGC26000.0125.000011
TTTGCTG152.1971202E-4125.000012
TGTTGCT26050.0124.999992
GTTGCTG26150.0124.521993
TTGCTGA26300.0123.811784
TGCTGAA26200.0123.56875
GCTGAAG26200.0123.56876
CTGAAGC26050.0123.320537
TGAAGCT25900.0123.310818
GAAGCTG25850.0123.307549
GAGGCAG23900.062.50000444-45
GGAGAGA150.003468259562.50000414-15
GCTGGAT150.003468259562.50000412-13
GCTGGAA451.4551915E-1162.50000412-13
TTTCATT23900.062.50000464-65
AGTGGAG150.003468259562.50000410-11
Produced by FastQC (version 0.11.5)