FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_IMMT_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_IMMT_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19123
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA1838996.1616901113842No Hit
CTGTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG230.12027401558332898No Hit
CTGTTGCTGAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG230.12027401558332898No Hit
TGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG200.1045861005072426No Hit
GTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAGG200.1045861005072426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCT18850.0124.6684342
CTGTTGC18850.0124.6684341
GTTGCTG18900.0124.3386153
TTGCTGA18900.0124.3386154
TGCTGAA18950.0124.010565
GCTGAAG19000.0123.684216
GAAGCTG18950.0123.680759
CTGAAGC18950.0123.680757
TGAAGCT18950.0123.680758
TTGTTAC302.1867163E-762.566-67
TAAATTC302.1867163E-762.558-59
AATTCTT302.1867163E-762.560-61
AAATTCT302.1867163E-762.558-59
TGTTTAA302.1867163E-762.554-55
TGTTACT302.1867163E-762.568-69
GTTTAAA302.1867163E-762.554-55
TCTTTTG302.1867163E-762.562-63
GTTACTT302.1867163E-762.568-69
TTTGTTA302.1867163E-762.566-67
TTAAATT302.1867163E-762.556-57
Produced by FastQC (version 0.11.5)