FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_HEATR1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_HEATR1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10543
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC995094.37541496727687No Hit
TCTCTTCTCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA990.9390116665085839No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGATGCATCATC420.3983685857915204No Hit
TTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA310.29403395617945555No Hit
TCTTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA230.2181542255524993No Hit
TCTCTTCTCCCCCAAACCTGTTTTCCAGCTGATCCACCAGAGGCATCATC160.15175946125391254No Hit
TCTCTTTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA150.14227449492554303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTCT10400.0125.02
TCTCTTC10400.0125.01
TTCTCCC10500.0123.809525
TCTTCTC10500.0123.809523
TCTCCCC10450.0123.803836
CCCCCAA10400.0123.798079
TCCCCCA10400.0123.798078
CTTCTCC10550.0123.222754
CTCCCCC10450.0123.205757
GAGCGTC10450.062.500004112-113
CTTTACT10450.062.50000466-67
CCAGCTG10450.062.50000424-25
CTGGGTA10350.062.50000482-83
CTCTCTC10450.062.50000460-61
CTCTTTA10450.062.50000464-65
TGTTTTC10350.062.50000418-19
AAGAGCG10450.062.500004110-111
TGCTGGG10450.062.50000480-81
GATCGGA10450.062.500004104-105
TCGGAAG10450.062.500004106-107
Produced by FastQC (version 0.11.5)