FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_HEATR1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_HEATR1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7457
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC703794.36770819364355No Hit
TCTCTTCTCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA630.8448437709534665No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGATGCATCATC270.36207590183719995No Hit
TCTTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA180.2413839345581333No Hit
TTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA160.2145634973850074No Hit
TCTCTTTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA130.17433284162531848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTCT7250.0125.02
TCTCTTC7250.0125.01
CTCCCCC7400.0122.466227
TTCTCCC7400.0122.466225
CCCCCAA7400.0122.466229
TCTCCCC7400.0122.466226
TCTTCTC7400.0122.466223
CTTCTCC7400.0122.466224
TCCCCCA7400.0122.466228
TTCTTCT206.7045074E-493.750011
GTATCAA7350.062.50000886-87
TGCTCTC7350.062.50000858-59
TCAAGTC201.3450309E-462.50000448-49
TTACTTC201.3450309E-462.50000468-69
CCTGCTC4850.062.50000456-57
ACTTCTA201.3450309E-462.50000470-71
TCTAAAA201.3450309E-462.50000474-75
TTCTAAA201.3450309E-462.50000472-73
CTAAAAT201.3450309E-462.50000474-75
ATCAAGT201.3450309E-462.50000448-49
Produced by FastQC (version 0.11.5)