FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_HEATR1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_HEATR1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC1115693.89782004881744No Hit
TCTCTTCTCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA900.757511993939904No Hit
TCTCTTCTCCCCCAAGCTGGGTATCAAAAAGGAAGATAGATCGGAAGAGC360.3030047975759616No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGATGCATCATC310.2609207979126336No Hit
TCTCTTTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA270.22725359818197124No Hit
TTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA250.21041999831664No Hit
TCTTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA250.21041999831664No Hit
TCTCTTCTCCCCCAAACCTGTTTTCCAGCTGATCCACCAGAGGCATCATC170.14308559885531522No Hit
TCTCTTCTCCCCCAAGCCTGTTTTACAGCTGATCCACCAGAGGCATCATC130.10941839912465282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCCA152.1667537E-4125.07
TCCCCAA152.1667537E-4125.08
TCTCTTC11650.0124.999991
CTCTTCT11700.0124.465812
TCTTCTC11700.0124.465813
CTTCTCC11700.0124.465814
TTCTCCC11750.0123.9361655
TCTCCCC11750.0123.9361656
CTCCCCC11600.0123.922427
CCCCCAA11700.0123.397449
TCCCCCA11650.0123.390558
TCTGCTC5400.062.50000456-57
GTCTTCT201.3615859E-462.50000452-53
AGTCTTC201.3615859E-462.50000452-53
TCTTCTG201.3615859E-462.50000454-55
AAGTCTT201.3615859E-462.50000450-51
CTTCTGC5400.062.50000454-55
TCAAGTC255.400647E-662.548-49
TTACTTC255.400647E-662.568-69
CCAGCTG11700.062.524-25
Produced by FastQC (version 0.11.5)