FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_HEATR1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_HEATR1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC998994.70939603678771No Hit
TCTCTTCTCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA780.7395467905565564No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGATGCATCATC350.3318479188394804No Hit
TCTTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA230.21807148952308714No Hit
TTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA220.20859012041338768No Hit
TCTCTTCTCCCCCAAACCTGTTTTCCAGCTGATCCACCAGAGGCATCATC180.1706646439745899No Hit
TCTCTTTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATCA120.11377642931639328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTCT10150.0125.02
TCTTCTC10300.0124.39323
CTTCTCC10300.0124.39324
TCTCTTC10200.0124.387261
TTCTCCC10400.0123.798075
TCTCCCC10350.0123.7922746
CTCCCCC10250.0123.780497
TCCCCCA10150.0123.768488
CCCCCAA10250.0122.5609749
AAGGCTT3950.062.50000450-51
GTCTTCT201.35797E-462.50000452-53
AGTCTTC201.35797E-462.50000452-53
TCTTCTG201.35797E-462.50000454-55
AAGTCTT201.35797E-462.50000450-51
TCAAGTC302.138986E-762.548-49
TCTGCTC4200.062.556-57
GGCTTCT4000.062.552-53
ATCAAGT302.138986E-762.548-49
CTTCTGC4200.062.554-55
CAAGTCT302.138986E-762.550-51
Produced by FastQC (version 0.11.5)