FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_FNDC3A_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_FNDC3A_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16983
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT832249.00194311959018No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC806247.471000412176885No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT360.21197668256491786No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGCT270.1589825119236884No Hit
GGACCTTCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT170.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCA152.1832493E-4125.000015
GGACCTT16700.0125.01
GACCTTC16700.0125.02
CCTTCCC16750.0124.626884
ACCTTCC16750.0124.626883
CCCCAGC16650.0124.624638
TCCCCAG16650.0124.624637
TTCCCCA16600.0124.62356
CTTCCCC16600.0124.62355
CCCAGCT16850.0123.516329
TCCCAGC206.856683E-493.749997
TTCCCAG206.856683E-493.749996
GTAGATC150.003457465462.50000484-85
ATCTCGG150.003457465462.50000488-89
AGCTTCT150.003457465462.50000412-13
GGCGCCG150.003457465462.50000498-99
CTCTTTC150.003457465462.50000416-17
CATTAAA150.003457465462.500004108-109
TTCTCTT150.003457465462.50000414-15
GATCTCG150.003457465462.50000488-89
Produced by FastQC (version 0.11.5)