FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_FNDC3A_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_FNDC3A_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10459
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC499747.77703413328234No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT491747.01214265226121No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGCT300.2868343053829238No Hit
GGACCTTCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT160.152978296204226No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT150.1434171526914619No Hit
GGACCTTCCCCAGCTTCTCTTTCCATACAAACCGTCCATTCAGATCGGAA140.13385600917869778No Hit
GGACTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGCT140.13385600917869778No Hit
GGACCTTCCCCAGCTTCTCTTTCCCTACAAACCGTCCATTCAGATCGGAA130.12429486566593365No Hit
GGACCTTCCCCAGCTTCATTCCATACAAACCGTCCATTCAGATCGGAAGA110.1051725786404054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT10300.0125.01
CCTTCCC10300.0125.04
ACCTTCC10300.0125.03
GACCTTC10300.0125.02
TTCCCCA10400.0123.798086
CTTCCCC10400.0123.798085
TCCCCAG10400.0123.798087
CCCAGCT10300.0123.786419
CCCCAGC10300.0123.786418
TTACAAG10250.062.518-19
AGAATTT10150.062.534-35
TTGAATA10250.062.526-27
GAATACA10250.062.528-29
TTTATAG10150.062.538-39
GCTTCAT10250.062.512-13
CAGCTTC10300.062.510-11
TTCATTA10200.062.49999614-15
GGATGCT5250.062.49999644-45
ACAGAAT10200.062.49999632-33
AATTTAT10100.062.49999636-37
Produced by FastQC (version 0.11.5)