FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_FNDC3A_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_FNDC3A_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22401
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT1481966.15329672782465No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC644828.7844292665506No Hit
GGACCTTCCCCAGCTTCTCTTTCCATACAAACCGTCCATTCAGATCGGAA480.21427614838623277No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT420.18749162983795367No Hit
GGACTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT360.16070711128967458No Hit
GGACCTTCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT270.12053033346725592No Hit
GGACCTTCCCCAGCTTCATTCCATACAAACCGTCCATTCAGATCGGAAGA260.11606624704254273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTT152.1926085E-4125.09
TCCCAGC152.1926085E-4125.07
TTCCCAG152.1926085E-4125.06
ACCTTCC22300.0125.03
GACCTTC22300.0125.02
CCCCAGT152.1926085E-4125.08
CTTCCCA152.1926085E-4125.05
GGACCTT22250.0124.999991
CCTTCCC22250.0124.999994
TTCCCCA22200.0124.4369356
CTTCCCC22200.0124.4369355
TCCCCAG22200.0124.4369357
CCCCAGC22050.0124.4331058
CCCAGCT22200.0123.592349
GGATGCT6400.062.50000444-45
AGCTTCT201.3748872E-462.50000412-13
AGGATGC6400.062.50000444-45
CTGACTC6400.062.50000450-51
TTCATTA21900.062.514-15
TAGGATG21800.062.542-43
Produced by FastQC (version 0.11.5)