FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_FNDC3A_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_FNDC3A_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43848
Sequences flagged as poor quality0
Sequence length131
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC1840141.96542601715015No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT1681738.352946542601714No Hit
GGACCTTCCCCAGCTTCTCTTTCCATACAAACCGTCCATTCAGATCGGAA8161.8609742747673783No Hit
GGACCTTCCCCAGCTTCTCTTTCCCTACAAACCGTCCATTCAGATCGGAA7081.6146688560481663No Hit
GGACCTTCCCCAGCTTCATTCCATACAAACCGTCCATTCAGATCGGAAGA5531.2611749680715199No Hit
GGACCTTCCCCAGCTTCATTCCCTACAAACCGTCCATTCAGATCGGAAGA5191.1836343732895458No Hit
GGACCTTCCCCAGCTCTTTCCATACAAACCGTCCATTCAGATCGGAAGAG4360.9943440977923736No Hit
GGACCTTCCCCAGCTCTTTCCCTACAAACCGTCCATTCAGATCGGAAGAG2840.6476920270023718No Hit
GGACCTTCCCCAGTCTTTCCATACAAACCGTCCATTCAGATCGGAAGAGC2610.5952380952380952No Hit
GGACCTTCCCCAGTCTTTCCCTACAAACCGTCCATTCAGATCGGAAGAGC2570.5861156723225689No Hit
GGACCTTCCCCAGCTTCATCTTTCCCTACAAACCGTCCATTCAGATCGGA2070.47208538587848936No Hit
GGACCTTCCCCAGCTTCATCTTTCCATACAAACCGTCCATTCAGATCGGA2070.47208538587848936No Hit
GGACCTTCCCCAGCTTCTTTCCATACAAACCGTCCATTCAGATCGGAAGA1710.3899835796387521No Hit
GGACCTTCCCCAGCTTTCTTTCCATACAAACCGTCCATTCAGATCGGAAG1670.3808611567232257No Hit
GGACCTTCCCCAGCTTTCTTTCCCTACAAACCGTCCATTCAGATCGGAAG1580.3603357051632914No Hit
GGACCTTCCCCAGCTTCTTTCCCTACAAACCGTCCATTCAGATCGGAAGA1490.33981025360335704No Hit
GGACCTTCCCCAGCTTCATTCTTTCCCTACAAACCGTCCATTCAGATCGG1480.3375296478744755No Hit
GGACCTTCCCCAGCTTCATTCTTTCCATACAAACCGTCCATTCAGATCGG1270.28963692756796205No Hit
GGACCTTCCCCAGCTTCTCTTTCCCATACAAACCGTCCATTCAGATCGGA1210.2759532931946725No Hit
GGACCTTCCCCAGCTCTTTCCCATACAAACCGTCCATTCAGATCGGAAGA1080.24630541871921183No Hit
GGACCTTCCCCAGCTTCATTTCTTTCCCTACAAACCGTCCATTCAGATCG1030.23490239007480387No Hit
GGACCTTCCCCAGTCTTTCCCATACAAACCGTCCATTCAGATCGGAAGAG1030.23490239007480387No Hit
GGACCTTCCCCAGCTTCATTTCTTTCCATACAAACCGTCCATTCAGATCG980.2234993614303959No Hit
GGACCTTCCCCAGCTTCATTCCCATACAAACCGTCCATTCAGATCGGAAG930.21209633278598794No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGTT610.13911694946177705No Hit
GGACCTTCCCCAGTTCATTACAAGCTTGAATACAGAATTTATAGGATGCT580.13227513227513227No Hit
GGACCTTCCCCAGCTTCTTTCCCATACAAACCGTCCATTCAGATCGGAAG520.11859149790184273No Hit
GGACCTTCCCCAGCTTCATCTTTCCCATACAAACCGTCCATTCAGATCGG440.10034665207079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTC600.0125.09
GGACCTC152.2068758E-4125.01
GGACCCT152.2068758E-4125.01
ACCCTCC152.2068758E-4125.03
GACCCTC152.2068758E-4125.02
GACCTCC152.2068758E-4125.02
CCCTCCC152.2068758E-4125.04
GGACTTC204.41074E-6124.999991
ACTTCCC204.41074E-6124.999993
TTCCCAG204.41074E-6124.999996
CCCCAGT700.0124.999998
GACTTCC204.41074E-6124.999992
CCTTCCC43200.0124.855334
ACCTTCC43200.0124.855333
GACCTTC43100.0124.854982
GGACCTT42950.0124.854481
CTTCCCC43200.0124.276635
TCCCCAG43150.0124.275797
TTCCCCA43100.0124.274936
CCCCAGC42550.0124.118688
Produced by FastQC (version 0.11.5)