FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DISL3_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DISL3_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21566
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC944043.7726050264305No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC707532.806269127330054No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGAAGAGCGTC256211.879810813317258No Hit
CAATTGCCCACACCAACTCCAGTCACCAACGCTTAAGTTTCCATCCAAGA3521.6321988314940183No Hit
CAATTGCCCACACCAACTCCAGTCACACAACGCTTAAGTTTCCATCCAAG2321.0757674116665121No Hit
CAATTGCCCACACCAACGCTTAATTTTCCATCCAAGATCGGAAGAGCGTC2120.9830288416952611No Hit
CAATTGCCCACACCAACTCCAGTCACAACGCTTAAGTTTCCATCCAAGAT1830.8485579152369471No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGACGAGCGTC960.445145135862005No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCCGCTCTTCTCACCC850.39413892237781695No Hit
CAATTGCCCACACCAACTCCAGTCACACGCTTAAGTTTCCATCCAAGATC530.24575721042381526No Hit
CAATTGCCCACACCAACGCTTAAGTTCCATCCAAGATCGGAAGAGCGTCG410.19011406844106463No Hit
CAATTGCCCACACCAACTCCAGTCACCGCTTAAGTTTCCATCCAAGATCG400.1854771399425021No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA320.14838171195400165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGC21250.0125.000011
ATTGCCA204.359472E-6125.000013
AATTGCC21250.0125.000012
ATTGCCC21050.0125.03
TGCCACA152.191459E-4125.05
TTGCCCA20950.0125.04
TTGCCAC152.191459E-4125.04
GCCCACA21000.0124.702386
CCCACAC21000.0124.702387
TGCCCAC21000.0124.702385
CACACCA21100.0124.1113749
CCACACC21250.0123.823538
GCCACAC206.882354E-493.750016
CTCCAGT800.062.50000416-17
GGTCATT403.5652192E-1062.50000498-99
ACTCCAG850.062.50000416-17
AACTCCA850.062.50000414-15
CGGGTCA403.5652192E-1062.50000496-97
GGGGGCC201.374296E-462.50000490-91
GCCGGCT201.374296E-462.50000494-95
Produced by FastQC (version 0.11.5)