FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DISL3_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DISL3_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC943548.16478635969166No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC715736.53581091428863No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGAAGAGCGTC15527.922813824084946No Hit
CAATTGCCCACACCAACTCCAGTCACCAACGCTTAAGTTTCCATCCAAGA1260.643218132625453No Hit
CAATTGCCCACACCAACGCTTAATTTTCCATCCAAGATCGGAAGAGCGTC1140.5819592628516004No Hit
CAATTGCCCACACCAACTCCAGTCACACAACGCTTAAGTTTCCATCCAAG1080.551329827964674No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGACGAGCGTC620.3165041604982388No Hit
CAATTGCCCACACCAACTCCAGTCACAACGCTTAAGTTTCCATCCAAGAT430.21951095002297208No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA390.1990913267650212No Hit
CAATTGCCCACACCAACGCTTAAGTTCCATCCAAGATCGGAAGAGCGTCG370.18888151513604573No Hit
CAATTGCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA260.13272755117668078No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCCA210.10720302210424218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGC19350.0125.01
AATTGCC19300.0125.02
CAATGCC152.1883627E-4124.999991
ATTGCCC19250.0124.999993
ATGCCCA152.1883627E-4124.999993
AATGCCC152.1883627E-4124.999992
TTGCCCA19250.0124.999994
TGCCCAC19400.0124.033515
GCCCACA19350.0124.0310066
CCCACAC19350.0124.0310067
CACACCA19350.0123.7080159
CCACACC19300.0123.704668
TCTCATC7200.062.50000442-43
CAGTCAC302.1880624E-762.49999620-21
GTCACCA150.003461447862.49999622-23
CCAGTCA302.1880624E-762.49999618-19
CTCCAGT302.1880624E-762.49999616-17
ACTCCAG302.1880624E-762.49999616-17
TCCAGTC302.1880624E-762.49999618-19
GAAGAGT150.003461447862.499996122-123
Produced by FastQC (version 0.11.5)