FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DISL3_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DISL3_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25400
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC1024240.32283464566929No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC814132.0511811023622No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGAAGAGCGTC322512.696850393700787No Hit
CAATTGCCCACACCAACTCCAGTCACCAACGCTTAAGTTTCCATCCAAGA5912.326771653543307No Hit
CAATTGCCCACACCAACTCCAGTCACAACGCTTAAGTTTCCATCCAAGAT4211.6574803149606299No Hit
CAATTGCCCACACCAACTCCAGTCACACAACGCTTAAGTTTCCATCCAAG2701.0629921259842519No Hit
CAATTGCCCACACCAACGCTTAATTTTCCATCCAAGATCGGAAGAGCGTC2370.9330708661417322No Hit
CAATTGCCCACACCAACTCCAGTCACACGCTTAAGTTTCCATCCAAGATC2360.9291338582677164No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGACGAGCGTC1080.42519685039370075No Hit
CAATTGCCCACACCAACTCCAGTCACCGCTTAAGTTTCCATCCAAGATCG1020.4015748031496063No Hit
CAATTGCCCACACCAACGCTTAAGTTCCATCCAAGATCGGAAGAGCGTCG530.20866141732283464No Hit
CAATTGCCCACACCAACTCCAGTCAACGCTTAAGTTTCCATCCAAGATCG510.20078740157480315No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCCA300.11811023622047244No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA300.11811023622047244No Hit
CAATTGCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA290.11417322834645668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGC25000.0125.01
ATTGCCC24950.0125.03
ATTGCCA152.1960525E-4125.03
AATTGCC25100.0124.999992
TTGCCCA25000.0124.754
GCCCACA25050.0124.5016
CCCACAC25050.0124.5017
TGCCCAC25000.0124.55
CACACCA25200.0124.0079359
CCACACC25200.0124.0079358
CAGTCAC1850.062.50000420-21
AGTCACC650.062.50000420-21
ACACGCT302.2013228E-762.524-25
GGGTCGC150.0034674362.588-89
GTCACCA550.062.522-23
GTCACAC550.062.522-23
GTCACAA600.062.522-23
CACACGC302.2013228E-762.524-25
TCAAGAT150.0034674362.596-97
CCAGTCA1950.062.518-19
Produced by FastQC (version 0.11.5)