FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DISL3_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DISL3_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31540
Sequences flagged as poor quality0
Sequence length131
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC1382643.83639822447685No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC891128.253012048192772No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGAAGAGCGTC398212.625237793278377No Hit
CAATTGCCCACACCAACTCCAGTCACCAACGCTTAAGTTTCCATCCAAGA6542.073557387444515No Hit
CAATTGCCCACACCAACTCCAGTCACACAACGCTTAAGTTTCCATCCAAG6001.9023462270133165No Hit
CAATTGCCCACACCAACTCCAGTCACAACGCTTAAGTTTCCATCCAAGAT5951.8864933417882055No Hit
CAATTGCCCACACCAACGCTTAATTTTCCATCCAAGATCGGAAGAGCGTC2960.9384908053265695No Hit
CAATTGCCCACACCAACTCCAGTCACACGCTTAAGTTTCCATCCAAGATC2510.7958148383005706No Hit
CAATTGCCCACACCAACTCCAGTCACCGCTTAAGTTTCCATCCAAGATCG1580.5009511731135067No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGACGAGCGTC1230.38998097653772984No Hit
CAATTGCCCACACCAACGCTTAAGTTCCATCCAAGATCGGAAGAGCGTCG610.19340519974635384No Hit
CAATTGCCCACACCAACTCCAGTCACGCTTAAGTTTCCATCCAAGATCGG510.1616994292961319No Hit
CAATTGCCCACACCAACTCCAGTCAACGCTTAAGTTTCCATCCAAGATCG380.12048192771084339No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA370.11731135066582118No Hit
CAATTGCCCACACAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCCA360.11414077362079898No Hit
CAATTGCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCCA330.1046290424857324No Hit
CAATTGCCCACACCAACGCTTAAGTTTCATCCAAGATCGGAAGAGCGTCG320.10145846544071022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGC31000.0125.000011
GCCCACA31100.0124.5980766
AATTGCC31050.0124.597432
TTGCCCA31050.0124.597434
TGCCCAC31000.0124.596795
ATTGCCC31000.0124.596793
CCCACAC31050.0124.396137
CACACCA31150.0123.996799
CCACACC31100.0123.995188
ACACGCT201.3792221E-462.50000424-25
GTCACAC950.062.50000422-23
CACACGC201.3792221E-462.50000424-25
TAGGAAA201.3792221E-462.500004118-119
TAGTGCA201.3792221E-462.50000474-75
TTGTAGA201.3792221E-462.50000476-77
TTTTCCA403.601599E-1062.50000424-25
CATCATT201.3792221E-462.50000498-99
CGGACGA201.3792221E-462.50000438-39
TCGGACG201.3792221E-462.50000438-39
ATAGTGC201.3792221E-462.50000472-73
Produced by FastQC (version 0.11.5)