FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DENND5A_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DENND5A_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14966
Sequences flagged as poor quality0
Sequence length131
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG565537.78564746759321No Hit
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA388925.98556728584792Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG260917.432847788320196No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG4513.013497260457036No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG1981.3229987972738206No Hit
CTGAGGTGAAGTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGG920.6147267138848056Illumina Single End PCR Primer 1 (100% over 24bp)
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGCC680.45436322330616064No Hit
CTGAGGTGAAGTGACTGGGTGTGATGAGGCAGATAGATCGGAAGAGCGTC470.31404516904984636No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG430.28731792062007216No Hit
CTGAGGTTAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA420.2806361085126286Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA410.2739542964051851Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGGGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA400.26727248429774153Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG360.2405452358679674No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG360.2405452358679674No Hit
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATGCC320.21381798743819325No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG270.18040892690097554No Hit
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG260.173727114793532No Hit
GAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAAAG250.16704530268608847Illumina Single End PCR Primer 1 (100% over 28bp)
CTGAGGTGAAGTGTAGGGAAAGAGGGTGTGATGAGGCAGATAGATCGGAA240.16036349057864493No Hit
CTGAGGTGAAGTGTAGGGAAAGAGTGTGATGAGGCAGATAGATCGGAAGA240.16036349057864493No Hit
CTGAGGTGAAGTGACTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGT210.1403180542563143No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG190.12695443004142723No Hit
CTGAGGTGAAGTGTAGGGAAAGAGGTGTGATGAGGCAGATAGATCGGAAG190.12695443004142723No Hit
CTGAGGTGAATTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA180.1202726179339837Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGCGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG170.11359080582654016No Hit
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG170.11359080582654016No Hit
CTGAGGTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGC150.10022718161165307No Hit
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG150.10022718161165307No Hit
CTGAGGCGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA150.10022718161165307Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGAAA150.10022718161165307Illumina Single End PCR Primer 1 (96% over 26bp)
CTGAGGTGAAGTGTAGGGAAAGAGTGATGAGGCAGATAGATCGGAAGAGC150.10022718161165307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGG258.569077E-8125.01
GAAGTGT258.569077E-8125.08
GGAAGTG258.569077E-8125.07
GGGAAGT258.569077E-8125.06
GGGGAAG258.569077E-8125.05
GGTTAAG204.315256E-6124.999995
TTAAGTG204.315256E-6124.999997
TGAGGTT204.315256E-6124.999992
TGAGGGG204.315256E-6124.999992
AAGTGTA204.315256E-6124.999999
GAGGGGA204.315256E-6124.999993
GTTAAGT204.315256E-6124.999996
GAGGTTA204.315256E-6124.999993
CTGAGGT14250.0124.56141
TGAGGTG14050.0124.110312
AAGTGAT4300.0123.546529
GAGGTGA14200.0122.799293
GAAGTGA14100.0122.783688
TGAAGTG14100.0122.783687
AGGTGAA14200.0122.359154
Produced by FastQC (version 0.11.5)