FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DENND5A_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DENND5A_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG465541.94827430837163No Hit
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA231920.897539875642064Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG204218.401369739569255No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG3523.1720284761647295No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG1461.315670902045598No Hit
CTGAGGTGAAGTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGG570.5136523384698567Illumina Single End PCR Primer 1 (100% over 24bp)
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGCC430.38749211498603225No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG390.3514463368477967No Hit
CTGAGGTGAAGTGACTGCCTCTCTGAAGCTTATACTCTAGCAGAAGTAAG310.27935478057132557No Hit
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG290.2613318915022078No Hit
CTGAGGGGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA280.25232044696764894Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA230.20726322429485444Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACGTGTAGGGAAAGAGGGTGTGATGAGGCAGATAGAT230.20726322429485444No Hit
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATGCC220.1982517797602956No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG220.1982517797602956No Hit
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG200.18022889069117778No Hit
CTGAGGCGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG200.18022889069117778No Hit
CTGAGGTGAAGTGACGTGTAGGGAAAGAGGTGTGATGAGGCAGATAGATC190.1712174461566189No Hit
CTGAGGTGAAGTGTAGGGAAAGAGGTGTGATGAGGCAGATAGATCGGAAG170.15319455708750113No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG170.15319455708750113No Hit
CTGAGGTGAAGTGACTGCCTTGAAATTCTGTGATGAGGCAGATAGATCGG160.14418311255294222No Hit
TGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGC160.14418311255294222No Hit
GAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAAAG140.12616022348382447Illumina Single End PCR Primer 1 (100% over 28bp)
CTGAGGTTAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA140.12616022348382447Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG140.12616022348382447No Hit
CTGAGGCGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA130.11714877894926556Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACTGCCGGTGCCTCCCATTCTTCCCCTCTGCAGGTGG120.10813733441470669No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG120.10813733441470669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGT10450.0125.01
TGAGGTG10350.0125.02
AAGTGAT2050.0125.09
GAGGTGA10400.0124.399033
AGGTGAA10500.0123.809524
GTGAAGT10600.0122.64156
GAAGTGA10800.0122.106488
TGAAGTG10650.0122.065737
GGTGAAG10600.0122.051885
AAGTGAC8900.0120.084279
CCGGATC201.3595489E-462.50000484-85
ACAAAAA201.3595489E-462.50000496-97
GGATCAT201.3595489E-462.50000486-87
CGGATCA201.3595489E-462.50000486-87
GCCGGAT201.3595489E-462.50000484-85
TAACAAA201.3595489E-462.50000494-95
CGCCGGA201.3595489E-462.50000482-83
CTTCATT150.003441509862.552-53
TTCATTA150.003441509862.552-53
ACTGCCG150.003441509862.514-15
Produced by FastQC (version 0.11.5)