FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DENND5A_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DENND5A_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18795
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG812543.229582335727585No Hit
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA412221.931364724660813Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG285215.174248470337856No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG6563.4902899707368986No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG2311.2290502793296088No Hit
CTGAGGTGAAGTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGG1110.590582601755786Illumina Single End PCR Primer 1 (100% over 24bp)
CTGAGGTGAAGTGACTGCCTCTCTGAAGCTTATACTCTAGCAGAAGTAAG860.4575685022612397No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG700.37243947858473No Hit
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGCC680.36179835062516624No Hit
CTGAGGTGAAGTGACGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA550.29263101888800214Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG530.2819898909284384No Hit
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG460.24474594306996542No Hit
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG430.22878425113061984No Hit
CTGAGGGGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA370.1968608672519287Illumina Single End PCR Primer 1 (100% over 26bp)
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATGCC350.186219739292365No Hit
CTGAGGTGAAGTGTAGGGAAAGAGGTGTGATGAGGCAGATAGATCGGAAG300.1596169193934557No Hit
CTGAGGTGAAGTGACTGCCTGTGATGAGGCAGATAGATCGGAAGAGCGTC290.15429635541367384No Hit
CTGAGGCGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG280.14897579143389197No Hit
CTGAGGTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGC270.14365522745411013No Hit
CTGAGGTGAAGTGACTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGT250.13301409949454643No Hit
CTGAGGTTAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA250.13301409949454643Illumina Single End PCR Primer 1 (100% over 26bp)
GAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAAAG240.12769353551476456Illumina Single End PCR Primer 1 (100% over 28bp)
CTGAGGTGAAGTGTAGGGAAAGAGGGTGTGATGAGGCAGATAGATCGGAA230.12237297153498271No Hit
CTGAGGTGAATTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA220.11705240755520085Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGCGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA220.11705240755520085Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG220.11705240755520085No Hit
CTGAGGTGAAGTGACTGCCTTGAAATTCTGTGATGAGGCAGATAGATCGG210.11173184357541899No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG210.11173184357541899No Hit
TGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGC210.11173184357541899No Hit
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG210.11173184357541899No Hit
CTGAGGTGAAGTGTAGGGAAAGATGTGATGAGGCAGATAGATCGGAAGAG190.10109071561585528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAA152.1869395E-4125.03
CTGAGGT17950.0125.01
TTAAGTG152.1869395E-4125.07
TGAGGTA152.1869395E-4125.02
AGGTTAA152.1869395E-4125.04
AAGTGTA258.6565706E-8125.09
GAAGTGT450.0124.999998
GAGGTTA204.3444816E-6124.999993
TGAGGTG17700.0124.2937852
AGGTGAA17800.0122.5421454
GGTGAAG17700.0122.528255
GTGAAGT17700.0122.528256
TGAAGTG17650.0122.521247
GAGGTGA18000.0122.222233
AAGTGAT3950.0121.835459
GAAGTGA17400.0121.4080358
AAGTGAC13900.0120.0539559
AGGGGAA251.3171446E-5100.02
TGAGGTT251.3171446E-5100.02
GAGGGGA251.3171446E-5100.01
Produced by FastQC (version 0.11.5)