FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_DENND5A_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_DENND5A_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252
Sequences flagged as poor quality0
Sequence length131
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG509235.728318832444565No Hit
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA330523.18972775750772Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG330123.16166152119001No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG3812.673309009261858No Hit
CTGAGGTGAAGTGACTGCCTGGTTGAGTTGCTCCAGCACGCTCCGACATG2351.6488913836654504No Hit
CTGAGGTGAAGTGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGG680.4771260174010665Illumina Single End PCR Primer 1 (100% over 24bp)
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATGCC490.343811394891945No Hit
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG390.2736458040976705No Hit
CTGAGGTTAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG340.23856300870053326No Hit
CTGAGGTTAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA340.23856300870053326Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGGGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG310.2175133314622509No Hit
CTGAGGTGAAGTGACTGCCTCTCTGAAGCTTATACTCTAGCAGAAGTAAG300.21049677238282344No Hit
CTGAGGTGAAGTGTAGGGAAAGAGGTGTGATGAGGCAGATAGATCGGAAG300.21049677238282344No Hit
CTGAGGTGAAGTGACGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA290.20348021330339602Illumina Single End PCR Primer 1 (100% over 26bp)
GAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAAAG290.20348021330339602Illumina Single End PCR Primer 1 (100% over 28bp)
GAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATGCC270.18944709514454114No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG260.18243053606511367No Hit
CTGAGGTTAAGTGACTGCCTGGTTGAGTTGCTGCAGCACGCTCCGACATG250.1754139769856862No Hit
CTGAGGTGAATTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG230.1613808588268313No Hit
CTGAGGGGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA230.1613808588268313Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGCGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA200.140331181588549Illumina Single End PCR Primer 1 (100% over 26bp)
CTGAGGTGAAGTGTAGGGAAAGAGGGTGTGATGAGGCAGATAGATCGGAA180.12629806342969407No Hit
CTGAGGCGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG160.11226494527083919No Hit
CTGAGGTGAATTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA150.10524838619141172Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGT13800.0125.01
AAGTGAT4000.0125.09
TGAGGTG13850.0124.0974662
GAGGTGA13950.0122.759853
AGGTGAA13950.0122.759854
GGTGAAG13900.0122.75185
TGAAGTG13900.0122.75187
GTGAAGT13900.0122.75186
GAAGTGA13800.0122.735518
AAGTGAC9850.0121.1928949
TGGTTGA850.062.50000420-21
GTTGAGT850.062.50000422-23
GGTTGAG850.062.50000420-21
CCAGCAC3750.062.50000432-33
CTCCAGC3750.062.50000430-31
TTGAGTT850.062.50000422-23
CTGGTTG850.062.50000418-19
TGCTCCA3750.062.50000428-29
TGAGTTG850.062.50000424-25
GTTGCTC3750.062.50000426-27
Produced by FastQC (version 0.11.5)