FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_CEP350_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_CEP350_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT1060488.56594003173808No Hit
ACAGGCTTGCATATCAGAGCTATAAGAGTCAGTCTGCCTTTTACATAACT3062.5557504384865948No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTCCATAACT1481.2361145911634512No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGGGACCAGGGGATC670.5595924162699407Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATTTGGGACCAGGGG640.5345360394220329Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGTTTGGGACCAGGG320.26726801971101644Illumina Single End PCR Primer 1 (95% over 24bp)
ACAGGCTTGATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG310.25891589409504717No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATGGGACCAGGGGAT220.1837467635513238Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC180.15033826108744675Illumina Single End PCR Primer 1 (96% over 33bp)
ACAGGCTTGCATATCAGAGCTAGAAGAGTCCGTCTGCCTTTTACATAACT160.13363400985550822No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGGCTGCCTTTTACATAACT140.1169297586235697No Hit
ACAGGCTTGCTATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG130.10857763300760044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCT11650.0125.01
AGGCTTG11750.0123.936173
GGCTTGC11750.0123.936174
CAGGCTT11700.0123.9316252
GCTTGCA11800.0123.411025
CTTGCAT11750.0123.404266
GCATATC11900.0122.899169
TGCATAT11800.0122.8813558
TTGCATA11850.0122.362887
ATCAGAG11900.062.50000412-13
ATATCAG11850.062.50000410-11
TAGAAGA11450.062.520-21
TGGTTTG11600.062.566-67
GTTTGTG302.1517735E-762.568-69
CTATAAG302.1517735E-762.520-21
AACTGTG11800.062.546-47
ATAAGAG302.1517735E-762.522-23
GCTAGAA11450.062.518-19
TCTGGTT8200.062.564-65
AGCTATA302.1517735E-762.518-19
Produced by FastQC (version 0.11.5)