FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_CEP350_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_CEP350_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10251
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT914889.24007413910839No Hit
ACAGGCTTGCATATCAGAGCTATAAGAGTCAGTCTGCCTTTTACATAACT1951.9022534386889083No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTCCATAACT1091.0633108964979026No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGGGACCAGGGGATC1020.9950248756218906Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGGCTTGCATATCAGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG440.4292264169349332Illumina Single End PCR Primer 1 (97% over 35bp)
ACAGGCTTGATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG260.2536337918251878No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGGACCAGGGGATCT210.2048580626280363Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATGGGACCAGGGGAT170.16583747927031509Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGGCTGCCTTTTACATAACT150.1463271875914545No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGTTTGGGACCAGGG140.1365720417520242Illumina Single End PCR Primer 1 (95% over 24bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGACCAGGGGATCTCAG110.1073066042337333Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG110.1073066042337333Illumina Single End PCR Primer 1 (100% over 35bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATTGGGACCAGGGGA110.1073066042337333Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTTG10100.0125.000013
CAGGCTT10100.0125.000012
ACAGGCT10150.0125.01
GGCTTGC10100.0124.3811954
GCTTGCA10100.0124.3811955
CTTGCAT10100.0124.3811956
TTGCATA10050.0124.378117
TGCATAT10050.0124.378118
GCATATC10050.0124.378119
ATAGGGG255.3745334E-662.5124-125
AGAGGGA150.003437938262.534-35
AAGAGGG150.003437938262.532-33
AGGGACC150.003437938262.536-37
CAGAGCG255.3745334E-662.514-15
TGTGGTT3700.062.564-65
CTTGTGG3700.062.562-63
CCCTTGT3700.062.560-61
GAGGGAC150.003437938262.534-35
AAAGAGG201.3571972E-462.49999632-33
ATATCAG10150.062.1921210-11
Produced by FastQC (version 0.11.5)