FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_CEP350_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_CEP350_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17469
Sequences flagged as poor quality0
Sequence length131
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT1535487.89283874291603No Hit
ACAGGCTTGCATATCAGAGCTATAAGAGTCAGTCTGCCTTTTACATAACT4262.438605529795638No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTCCATAACT2221.2708226000343466No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGGGACCAGGGGATC1350.7727975270479135Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATTTGGGACCAGGGG870.49802507298643306Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG490.2804968801877612No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGTTTGGGACCAGGG430.24615032343007615Illumina Single End PCR Primer 1 (95% over 24bp)
ACAGGCTTGCATATCAGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGC350.20035491441982942Illumina Single End PCR Primer 1 (96% over 33bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATGGGACCAGGGGAT350.20035491441982942Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGATTGGGACCAGGGGA340.19463048829354856Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGGCTGCCTTTTACATAACT270.1545595054095827No Hit
ACAGGCTTGCATATCAGAGCGTCGTGTAGGGAAAGAGGACCAGGGGATCT260.14883507928330184Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGGCTTGCATATCAGAGCTAGAAGAGTCCGTCTGCCTTTTACATAACT240.13738622703074016No Hit
ACAGGCTTGCTATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG210.12021294865189765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATC152.1843503E-4125.000018
GGCTTGA152.1843503E-4125.000014
TTGATAT152.1843503E-4125.000017
GCTTGCA16850.0125.000015
GCTTGAT152.1843503E-4125.000015
CTTGATA152.1843503E-4125.000016
CCAGGCT152.1843503E-4125.000011
GGCTTGC16900.0125.04
CAGGCTT17100.0125.02
ACAGGCT17000.0125.01
AGGCTTG17050.0124.999993
CTTGCAT16900.0124.630186
TTGCATA17000.0124.26477
TGCATAT17050.0123.900288
GCATATC17000.0123.897069
CTTTTCC403.5288394E-1062.50000438-39
TGTGACC403.5288394E-1062.50000472-73
GTTTGTG403.5288394E-1062.50000468-69
CTATAAG403.5288394E-1062.50000420-21
ATAAGAG451.4551915E-1162.50000422-23
Produced by FastQC (version 0.11.5)