FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_CEP350_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_CEP350_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530
Sequences flagged as poor quality0
Sequence length131
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT1616592.21334854535083No Hit
ACAGGCTTGCATATCAGAGCTATAAGAGTCAGTCTGCCTTTTACATAACT4472.549914432401597No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTCCATAACT2341.3348545350827155No Hit
ACAGGCTTGATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG440.2509982886480319No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGGCTGCCTTTTACATAACT230.13120365088419852No Hit
AAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG210.11979463776383344No Hit
ACAGGCTTGCTATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG200.11409013120365087No Hit
ACAGGCTTGCATATAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACTG190.10838562464346835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAT17100.0125.000016
TTGCATA17150.0125.07
AGGCTTG17250.0125.03
GGCTTGC17250.0125.04
CAGGCTT17250.0125.02
GCTTGCA17200.0124.999995
ACAGGCT17300.0124.999991
TGCATAT17200.0124.636638
GCATATC17150.0124.635579
CTTTTCC302.1813503E-762.50000438-39
GTTTGTG150.00345840662.50000468-69
CTATAAG700.062.50000420-21
ATAAGAG700.062.50000422-23
TTTCCAT302.1813503E-762.50000440-41
AGCTATA700.062.50000418-19
TATAAGA700.062.50000420-21
TTTTCCA302.1813503E-762.50000438-39
GGTTTGT150.00345840662.50000468-69
TAAGAGT700.062.50000422-23
GAGCTAT700.062.50000416-17
Produced by FastQC (version 0.11.5)