FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68724
Sequences flagged as poor quality0
Sequence length131
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA39645.767999534369361No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30644.458413363599325No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG29614.308538501833421No Hit
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC26573.866189395262208No Hit
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA25623.7279552994587046No Hit
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG23853.4704033525405973No Hit
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG23383.402013852511495No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT18862.7443105756358768No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG14612.1258948838833596No Hit
GAGATCCCCTGGTCCCAAACCACAAGGGCTACTGGCACAGTTATGTAAAA13621.9818404050986556No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC13301.9352773412490543No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG12931.8814387986729526No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT10581.5394912985274432No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG8891.2935801175717363No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT8701.2659332984110354No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC7361.07095046854083No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGC4470.6504277981491182No Hit
TGGATGGAAACTTAAGCGTTGGTGTGGGCAATTGAGATCGGAAGAGCACA3410.4961876491473139No Hit
ATCTGCCTCATCACTTCACCTCAGAGATCGGAAGAGCACACGTCTGAACT2930.42634305337291195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG2640.38414527675921073No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCT2600.3783248937780106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATTGTCG2260.3288516384378092No Hit
CAGTCGGGTCCACTAAAAATCTCTCTTTTCTCCTCCTTCCTTCCCTCCTC1580.22990512775740643No Hit
GAATGGACGGTTTGTATCCCTATCCCTCTTCCCCTTTCCTCCCTCCCCCT1370.19934811710610562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1100.1600605319830045No Hit
GAGTGACAGCGAAGAAGAATTTTTTTCCTTCCTCCTTTCTCCTTCCTCCC840.12222804260520342No Hit
TGGATGGAAACTTAAGCGTTGTTTCTTCTCTTCCCTCCTTCCCCTCCTTT830.12077294685990338No Hit
TCTTCCTTCTAATGATGAGCCCCTCCTTTCCTTTTCCCCTCCCCCCTCCT690.1004016064257028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGA3800.0125.000012
CCTTCTA3800.0125.000015
TGTTGTT3450.0125.07
GTGTTGT3450.0125.06
GGGTCCA3100.0125.06
TCAGGTG152.2123035E-4125.04
GAGTGAC1350.0125.01
GGTCCAC3100.0125.07
TGCTGTC152.2123035E-4125.09
GGTTTGT4000.0125.09
CTGGTCC4400.0125.09
CGGGTCC3100.0125.05
AGTGTTG3450.0125.05
TATCTCA4200.0125.06
AGAAAGT3500.0125.01
GGCTCAG152.2123035E-4125.01
CTCAGGT152.2123035E-4125.03
TGGACGG4000.0125.04
CCCCTGG4400.0125.06
CGGTTTG4000.0125.08
Produced by FastQC (version 0.11.5)