FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RNPEP_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RNPEP_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12980
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA1224594.33744221879815No Hit
CCAAGTATCTCAAATGCCCGGAATTCAGAGCTCCGGCTGCGATGGGGCCA930.7164869029275809No Hit
CAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA530.40832049306625573No Hit
CCAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA500.3852080123266564No Hit
CCAAGTATCTCAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA330.2542372881355932No Hit
CCAAGTATCTAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA200.15408320493066258No Hit
CCAAGTATTTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA160.12326656394453005No Hit
CCAAGATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA140.10785824345146379No Hit
CCAAGTATCTCAAATGCCTGGAAAGTGAAGGAGTAGATCGGAAGAGCACA140.10785824345146379No Hit
CTAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA130.10015408320493066No Hit
CCAAGTTCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA130.10015408320493066No Hit
CCAAGTATTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA130.10015408320493066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGTA12600.0125.01
AAGTATC12700.0124.015753
CAAGTAT12700.0124.015752
ATCTCAA12850.0123.0544747
TATCTCA12850.0123.0544746
GTATCTC12800.0123.046885
AGTATCT12800.0123.046884
TCTCAAA12800.0123.046888
CTCAAAT12850.0122.568099
AGAGCAC12650.062.500004108-109
AGTAATC358.6402E-962.50000498-99
GAGTAAT358.6402E-962.50000496-97
TAATCGG358.6402E-962.500004100-101
GCAGAGC12800.062.50000424-25
ATGCAGA12800.062.50000422-23
AAGTGAA12800.062.50000488-89
GTAATCG358.6402E-962.50000498-99
AATCGGA358.6402E-962.500004100-101
GAAAGTG12800.062.50000486-87
GGAGTAA358.6402E-962.50000496-97
Produced by FastQC (version 0.11.5)