FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RNPEP_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RNPEP_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19159
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA1800493.97150164413591No Hit
CCAAGTATCTCAAATGCCCGGAATTCAGAGCTCCGGCTGCGATGGGGCCA1330.6941907197661673No Hit
CCAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA770.40189989039093904No Hit
CAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA690.36014405762304924No Hit
CCAAGTATCTCAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA470.2453155175113524No Hit
CCAAGTATCTCAAATGCCTGGAAAGTGAAGGAGTAGATCGGAAGAGCACA360.18790124745550393No Hit
CCAAGTATCTAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA290.1513648937836004No Hit
CCAATATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA260.13570645649564175No Hit
CCAAGTATTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA250.1304869773996555No Hit
CCAAGTTCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA240.1252674983036693No Hit
CCAAGTATCTCAAATGCCCGTAATGCAGAGCTCCGGCTGCGATGGGGCCA220.11482854011169687No Hit
CCAAGTATCTCAAATGCCCTGAATGCAGAGCTCCGGCTGCGATGGGGCCA210.10960906101571063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGTA18800.0125.000011
AAGTATC18900.0124.338633
CAAGTAT18900.0124.338632
AGTATCT18900.0124.007944
GTATCTC18850.0124.005315
TATCTCA18850.0124.005316
ATCTCAA18900.0123.677257
TCTCAAA18900.0123.3465658
CTCAAAT18950.0123.021129
GATGGGG18950.062.50000440-41
GGAGAGC201.3723449E-462.500004106-107
AATCCAG201.3723449E-462.500004122-123
AGCTCCG18850.062.50000428-29
TGACTCC358.789357E-962.500004120-121
GCGATGG18950.062.50000438-39
CCCGGAA18850.062.50000416-17
GAACGAC18950.062.50000462-63
ATGAAGG201.3723449E-462.50000490-91
CTGACTC358.789357E-962.500004120-121
GTCTGAC358.789357E-962.500004118-119
Produced by FastQC (version 0.11.5)