FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RNPEP_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RNPEP_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12062
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA1130493.71580169126182No Hit
CCAAGTATCTCAAATGCCCGGAATTCAGAGCTCCGGCTGCGATGGGGCCA780.6466589288675179No Hit
CCAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA530.4393964516663903No Hit
CCAAGTATCTCAAATGCCTGGAAAGTGAAGGAGTAGATCGGAAGAGCACA410.33991046260984914No Hit
CAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA400.331619963521804No Hit
CCAAGTATCTCAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA220.18239097993699221No Hit
CCAAGTTCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA210.1741004808489471No Hit
CCAAGTATCTAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA200.165809981760902No Hit
CCAAGATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA200.165809981760902No Hit
CCAAGTATTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA170.1409384844967667No Hit
TCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA140.11606698723263141No Hit
CTAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA130.1077764881445863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATC11800.0125.03
GTATCTC11800.0125.05
AGTATCT11800.0125.04
CCAAGTA11800.0125.01
CAAGTAT11850.0125.02
ATCTCAA11900.0124.4747857
TATCTCA11850.0124.472576
TCTCAAA11950.0123.430968
CTCAAAT11950.0123.430969
GGAGAGC358.605639E-962.500004106-107
TCGGAGA358.605639E-962.500004104-105
ATCGGAG358.605639E-962.500004102-103
AATCGTC8600.062.50000450-51
CAAATCG8650.062.50000448-49
TTTTGGA150.003445310362.582-83
CGGAGAG302.1525375E-762.5104-105
TGACTCC255.403428E-662.5120-121
TTTTTGG150.003445310362.580-81
CTGACTC255.403428E-662.5120-121
GTCTGAC255.403428E-662.5118-119
Produced by FastQC (version 0.11.5)