FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RNPEP_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RNPEP_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16981
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA1591093.69295094517402No Hit
CCAAGTATCTCAAATGCCCGGAATTCAGAGCTCCGGCTGCGATGGGGCCA1070.6301160120134268No Hit
CCAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA680.4004475590365703No Hit
CAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA630.371002885577999No Hit
CCAAGTATCTCAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA460.2708909958188564No Hit
CCAAGTATTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA360.2120016489017137No Hit
CCAAGTATCTCAAATGCCTGGAAAGTGAAGGAGTAGATCGGAAGAGCACA360.2120016489017137No Hit
CCAAGTATCTAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA290.1707791060597138No Hit
CCAAGTATTTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA240.14133443260114245No Hit
CCAAGTATCTCAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCTG210.12366762852599965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAAGTTCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA190.11188975914257111No Hit
TCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA180.10600082445085685No Hit
CCAATATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAA170.10011188975914258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGTA16600.0125.000011
AAGTATC16700.0124.25153
CAAGTAT16700.0124.25152
AGTATCT16800.0123.511914
GTATCTC16750.0123.507475
ATCTCAA16700.0123.5037
TATCTCA16700.0123.5036
TCTCAAA16650.0123.123138
CTCAAAT16600.0123.117489
GATGGGG16650.062.50000440-41
GCGATGG16650.062.50000438-39
AAGAGTA150.003457465462.50000494-95
CTCCATC150.003457465462.500004124-125
GTCTGAC302.1792766E-762.500004118-119
GAAGAGT150.003457465462.50000492-93
GAAGACA302.1792766E-762.500004106-107
ACACACG302.1792766E-762.500004110-111
GACACAC302.1792766E-762.500004110-111
GTCCTTA10100.062.50000454-55
TGGGGCC16650.062.50000442-43
Produced by FastQC (version 0.11.5)