FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RAB3GAP1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RAB3GAP1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8952
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC838093.61036639857015No Hit
GAGTGACAGCGAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT480.5361930294906166No Hit
AGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT330.3686327077747989No Hit
GAGTGACAGCGAAGAAGAATTTTTTTGAATGCCTAAGTGATACTGAAGAA280.3127792672028597No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG270.30160857908847183No Hit
GGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT200.22341376228775692No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG200.22341376228775692No Hit
GAGTGAAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT170.18990169794459338No Hit
GAGTGACAGCGAAGAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT170.18990169794459338No Hit
GAGTGACGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT150.1675603217158177No Hit
GAGTGACACGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT150.1675603217158177No Hit
GAGTGACAGCAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT150.1675603217158177No Hit
GATGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT110.12287756925826632No Hit
GAGTACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT110.12287756925826632No Hit
GAGTGACAGCGAAAAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC100.11170688114387846No Hit
GAGTGACAGGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT90.10053619302949061No Hit
GAGTGCAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT90.10053619302949061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGA8900.0125.06
GAGTGAC8900.0125.01
GACAGCG8850.0125.05
TGACAGC8850.0125.04
GCGAAGA8800.0125.09
AGTGACA8900.0125.02
GTGACAG8900.0125.03
CAGCGAA8850.0125.07
AGCGAAG8800.0125.08
GAAATGG5550.062.50000456-57
AGTGGCA8250.062.50000470-71
AGGAAAT5550.062.50000454-55
GGACAAT900.062.50000462-63
CAATAGA900.062.50000464-65
AGAGTGG8250.062.50000468-69
AATAGAG451.2732926E-1162.50000466-67
GACAATA900.062.50000462-63
AATGGAC5550.062.50000458-59
ATAGAGT451.2732926E-1162.50000466-67
TAGAGTG451.2732926E-1162.50000468-69
Produced by FastQC (version 0.11.5)