FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RAB3GAP1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RAB3GAP1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC1052392.76269393511988No Hit
GAGTGACAGCGAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT530.4672073342736248No Hit
AGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT430.3790550070521862No Hit
GGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT400.3526093088857546No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG370.326163610719323No Hit
GAGTGACAGCGAAGAAGGACCTAAGGAGATGGCAGATCGGAAGAGCACAC320.2820874471086037No Hit
GAGTGACAGCGAAGAAGAATTTTTTTGAATGCCTAAGTGATACTGAAGAA220.19393511988716503No Hit
GAGTGACGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT220.19393511988716503No Hit
GAGTGACAGGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT200.1763046544428773No Hit
GAGTGAAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT180.15867418899858957No Hit
GAGTACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT170.14985895627644572No Hit
GAGTGACAGCGAAGAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT150.13222849083215796No Hit
GAGGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT140.1234132581100141No Hit
GAGTGACACGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT130.11459802538787023No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG130.11459802538787023No Hit
GAGTGACAGCGAAGAAAAATTTTTTGAATGCCTAAGTGATACTGAAGAAC130.11459802538787023No Hit
GAGTGACAGCAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT120.10578279266572638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC10950.0125.01
TGACAGC11150.0123.878924
GTGACAG11150.0123.878923
ACAGCGA11100.0123.873886
GACAGCG11100.0123.873885
CAGCGAA11100.0123.873887
AGCGAAG11050.0123.8687748
GCGAAGA11100.0123.3108149
AGTGACA11200.0122.767862
ACATGGA403.43789E-1062.50000458-59
AGGACGT358.572897E-962.50000454-55
AGGACAT403.43789E-1062.50000454-55
ATTGGCA451.4551915E-1162.50000470-71
GGACATG403.43789E-1062.50000456-57
GGACAAT800.062.50000462-63
GAAGAAG11100.062.50000410-11
GATTGGC451.4551915E-1162.50000470-71
GACATGG403.43789E-1062.50000456-57
CGTGGAC358.572897E-962.50000458-59
GACGTGG358.572897E-962.50000456-57
Produced by FastQC (version 0.11.5)