FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RAB3GAP1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RAB3GAP1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16001
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC1442790.16311480532467No Hit
GAGTGACAGCGAAGAAGAATTTTTGAATGCCTAAGTGATACTGAAGAACT1330.8311980501218674No Hit
GAGTGACAGCGAAGAAGAATTTGAATGCCTAAGTGATACTGAAGAACTTA1270.7937003937253921No Hit
GAGTGACAGCGAAGAAGAATTTTGAATGCCTAAGTGATACTGAAGAACTT1140.7124554715330291No Hit
GAGTGACAGCGAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT750.4687207049559402No Hit
GGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT460.2874820323729767No Hit
AGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT420.26248359477532657No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG330.20623711018061372No Hit
GAGTGAAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT320.19998750078120117No Hit
GAGTGACAGCGAAGAAGAATTTTTTTGAATGCCTAAGTGATACTGAAGAA310.19373789138178865No Hit
GAGTGACAGCGAAGAAGGACCTAAGGAGATGGCAGATCGGAAGAGCACAC280.17498906318355104No Hit
GAGTGACAGCGAAGAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT230.14374101618648835No Hit
GAGTGACAGCGAAGAAGAATTGAATGCCTAAGTGATACTGAAGAACTTAA230.14374101618648835No Hit
GAGTGACAGGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT230.14374101618648835No Hit
GAGTACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT230.14374101618648835No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG210.13124179738766328No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG210.13124179738766328No Hit
GAGTGACAGCAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT210.13124179738766328No Hit
GAGTGACAGCGAAAAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC200.12499218798825074No Hit
GAGTGACAGCGAAGAAAAATTTTTTGAATGCCTAAGTGATACTGAAGAAC200.12499218798825074No Hit
GAGTGACGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT190.11874257858883819No Hit
GAGTGACAGCGAAAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT180.11249296918942565No Hit
GAGTGACACGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT180.11249296918942565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAA204.3247037E-6125.000016
GAGTGAC15550.0125.000011
GACAGCA204.3247037E-6125.000015
CAGCAAG152.1809698E-4125.07
AGCAAGA152.1809698E-4125.08
CAGCGAG152.1809698E-4125.07
AGCGAGA152.1809698E-4125.08
GCGAGAA152.1809698E-4125.09
AGTGACA15600.0124.5993652
TGACAGC15550.0124.5980764
GTGACAG15550.0124.5980763
GACAGCG15400.0124.188315
ACAGCGA15550.0123.392296
CAGCGAA15400.0123.3766257
GCGAAGA15500.0122.580649
AGCGAAG15500.0122.580648
ATTGGCA700.062.50000470-71
GGACAAT1600.062.50000462-63
GAAGAAG15450.062.50000410-11
GATTGGC700.062.50000470-71
Produced by FastQC (version 0.11.5)