FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_RAB3GAP1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_RAB3GAP1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16055
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC1476191.9402055434444No Hit
GAGTGACAGCGAAGAAGAATTTTTGAATGCCTAAGTGATACTGAAGAACT1400.8720024914356898No Hit
GAGTGACAGCGAAGAAGAATTTTGAATGCCTAAGTGATACTGAAGAACTT1270.7910308315166614No Hit
GAGTGACAGCGAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT730.45468701339146683No Hit
GAGTGACAGCGAAGAAGAATTTGAATGCCTAAGTGATACTGAAGAACTTA480.2989722827779508No Hit
GAGTGACAGCGAAGAAGAATTTTTTTGAATGCCTAAGTGATACTGAAGAA480.2989722827779508No Hit
AGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT440.2740579258797882No Hit
GAGTGACAGCGAAGAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT360.22422921208346308No Hit
GGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT360.22422921208346308No Hit
GAGTGAAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT330.20554344440984118No Hit
GAGTGACAGGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT330.20554344440984118No Hit
GAGTACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT320.19931485518530054No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG260.16194331983805668No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG240.1494861413889754No Hit
GAGTGACAGCAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT240.1494861413889754No Hit
GAGTGACGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT230.14325755216443475No Hit
GAGTGCAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT230.14325755216443475No Hit
GATGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT210.13080037371535347No Hit
GAGTGACAGCGAAGAAAAATTTTTTGAATGCCTAAGTGATACTGAAGAAC200.12457178449081284No Hit
GAGTGACACGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT180.11211460604173154No Hit
GAGGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAACT180.11211460604173154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC15350.0125.000011
GGTGACA152.1810965E-4125.01
AGTGACA15500.0123.790322
GTGACAG15550.0123.392293
TGACAGC15450.0123.381884
ACAGCGA15500.0122.580646
GACAGCG15500.0122.580645
CAGCGAA15500.0122.177427
AGCGAAG15600.0120.993588
GCGAAGA15650.0120.607039
ACATGGA800.062.50000458-59
AGGACGT403.5106495E-1062.50000454-55
AGGACAT800.062.50000454-55
GGACATG800.062.50000456-57
GGACAAT1600.062.50000462-63
GACATGG800.062.50000456-57
CAATAGA1600.062.50000464-65
CGTGGAC403.5106495E-1062.50000458-59
GACGTGG403.5106495E-1062.50000456-57
GACAATA1600.062.50000462-63
Produced by FastQC (version 0.11.5)