FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_QSER1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_QSER1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17514
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG1101562.89254310837045No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG549731.38631951581592No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC510.29119561493662216No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT500.28548589699668836No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG430.24551787141715198No Hit
AAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT390.22267899965741694No Hit
GAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT290.16558182025807924No Hit
AGAAAGTGTTGTTGGTTCAATGACACAACTTAACCAACAAATTGGCCAAG230.13132351261847663No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT200.11419435879867536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAGT17250.0125.01
GAAAGTG17250.0125.02
AAAGTGT17350.0124.279543
GTGTTGT17450.0123.567336
AGTGTTG17450.0123.567335
AAGTGTT17450.0123.567334
GTTGTTG17400.0123.5632258
TTGTTGG17300.0123.554919
TGTTGTT17500.0123.214287
AATCCAG150.00345840662.500004102-103
ATCTCGG150.00345840662.500004122-123
ATCTTAG150.00345840662.50000472-73
TGATCTA201.3706944E-462.50000470-71
CTTAGAA150.00345840662.50000474-75
GAAGACG700.062.50000478-79
GAAGACA201.3706944E-462.50000486-87
GACACAC201.3706944E-462.50000490-91
CTGAATC150.00345840662.500004100-101
AAGACAC201.3706944E-462.50000488-89
AGACGGA700.062.50000480-81
Produced by FastQC (version 0.11.5)