FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_QSER1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_QSER1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16389
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG1017962.108731466227354No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG516531.515040575996096No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC580.3538959057904692No Hit
GAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT510.3111843309536884No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT430.26237110256879614No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG420.2562694490206846No Hit
AAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT380.23186283482823844No Hit
GAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT360.21965952773201539No Hit
AGAAAGTGTTGTTGGTTCAGTTACACAACTTAACCAACAAATTGGCCAAG190.11593141741411922No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT180.10982976386600769No Hit
AGAAAGTGTTGTTGGTTCAATGACACAACTTAACCAACAAATTGGCCAAG170.10372811031789614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAGT15950.0124.608151
GAAAGTG16100.0123.83542
AAAGTGT16250.0123.076923
TGTTGTT16200.0122.685197
GTGTTGT16200.0122.685196
AGTGTTG16200.0122.685195
AAGTGTT16200.0122.685194
TTGTTGG16250.0122.3076869
GTTGTTG16250.0122.3076868
CGCGAAT900.062.500004116-117
CGACAAA5600.062.50000434-35
ATCGGAG358.734787E-962.50000482-83
GACAAAT5600.062.50000436-37
CGAATCT900.062.500004118-119
ACAAATT16000.062.536-37
AACGTCT150.003456368362.594-95
AATCCAG150.003456368362.5102-103
CGGAGAG255.4469183E-662.584-85
ATCCAGT150.003456368362.5104-105
TCGAAGA150.003456368362.584-85
Produced by FastQC (version 0.11.5)