FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_QSER1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_QSER1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42783
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG1048624.509735175186407No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG888820.7746067363205No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG643315.036346212280579No Hit
AGAAAGTGTTGTTGGTTCAGTACCCTTGATCTTAAGAAGATCGGAAGAGC14523.3938713975177057No Hit
AGAAAGTGTTGTTGGTTCAGTCACCAGCTACCCTTGATCTTAAGAAGATC12853.0035294392632585No Hit
AGAAAGTGTTGTTGGTTCAGTGCAGCTACCCTTGATCTTAAGAAGATCGG9722.271930439660613No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAATAG4491.049482270995489No Hit
AGAAAGTGTTGTTGGTTCAGTCACTGCAGCTACCCTTGATCTTAAGAAGA4471.0448075170044175No Hit
AGAAAGTGTTGTTGGGAAAGACAGCTACCCTTGATCTTAAGAAGATCGGA3910.9139144052544235No Hit
AGAAAGTGTTGTTGGTTCAGTCACGCAGCTACCCTTGATCTTAAGAAGAT3460.808732440455321No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGACGGAAGAGC3420.7993829324731786No Hit
AGAAAGTGTTGTTGGTTCAGTGCCCTTGATCTTAAGAAGATCGGAAGAGC2710.6334291657901503No Hit
AGAAAGTGTTGTTGGGAAAGAGCAGCTACCCTTGATCTTAAGAAGATCGG2380.5562957249374753No Hit
AGAAAGTGTTGTTGGTTCAGTTACCCTTGATCTTAAGAAGATCGGAAGAG2300.5375967089731903No Hit
AGAAAGTGTTGTTGGTTCAGTCACCTACCCTTGATCTTAAGAAGATCGGA2220.5188976930089054No Hit
AGAAAGTGTTGTTGGGGAAAGACAGCTACCCTTGATCTTAAGAAGATCGG2060.4814996610803357No Hit
AGAAAGTGTTGTTGGTTCAGTCACAGCTACCCTTGATCTTAAGAAGATCG1640.38332982726784004No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAGAGC1300.3038590094196293No Hit
AGAAAGTGTTGTTGGTTCAGTCACGCTACCCTTGATCTTAAGAAGATCGG1170.27347310847766637No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC1120.2617862234999883No Hit
AGAAAGTGTTGTTGGGAAAGACTACCCTTGATCTTAAGAAGATCGGAAGA1040.24308720753570343No Hit
AGAAAGTGTTGTTGGGGAAAGAGCAGCTACCCTTGATCTTAAGAAGATCG1020.23841245354463222No Hit
AGAAAGTGTTGTTGGTGCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG950.22205081457588294No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAGATCGGAAGAGC810.18932753663838442No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAC770.17997802865624196No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAAGC690.16127901269195707No Hit
AGAAAGTGTTGTTGGGAAAGAGCTACCCTTGATCTTAAGAAGATCGGAAG630.14725475071874342No Hit
AGAAAGTGTTGTTGGGAAAGATGCAGCTACCCTTGATCTTAAGAAGATCG600.1402426197321366No Hit
AGAAAGTGTTGTTGGTAGGGAAAGACAGCTACCCTTGATCTTAAGAAGAT570.13323048874552976No Hit
AGAAAGTGTTGTTGGTTCAGTGTGCAGCTACCCTTGATCTTAAGAAGATC560.13089311174999416No Hit
AGAAAGTGTTGTTGCAGCTACCCTTGATCTTAAGAAGATCGGAAGAGCAC550.12855573475445856No Hit
AGAAAGTGTTGTTGGTTCAGTACCCTTGATCTTAAGAAGACGGAAGAGCA520.12154360376785171No Hit
AGAAAGTGTTGTTGGTAAAGACAGCTACCCTTGATCTTAAGAAGATCGGA520.12154360376785171No Hit
AGAAAGTGTTGTTGGTTCAGTGAAAGAGCAGCTACCCTTGATCTTAAGAA490.1145314727812449No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTAAGAAGATCGGAAGAGC470.10985671879017367No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAATCGGAAGAGC470.10985671879017367No Hit
AGAAAGTGTTGTTGGTTCAGTCACCAGCTACCCTTGATCTTAAGAAGACG460.10751934179463805No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT460.10751934179463805No Hit
AGAAAGTGTTGTTGGTTCAGTGAAAGACAGCTACCCTTGATCTTAAGAAG450.10518196479910244No Hit
AGAAAGTGTTGTTGGTGAAAGACAGCTACCCTTGATCTTAAGAAGATCGG440.10284458780356683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAGT41950.0125.01
GAAAGTG42200.0124.2594762
AAAGTGT42300.0123.670223
TGTTGTT42350.0123.3766257
GTGTTGT42300.0123.374716
AGTGTTG42300.0123.374715
AAGTGTT42300.0123.374714
GTTGTTG42400.0123.2311258
TTGTTGG42350.0123.229049
AAAAGTG250.001681021875.01
TGTTGGG1950.062.50000410-11
GTCACCA1550.062.50000420-21
CCAGCTA1550.062.50000424-25
GTACCCT1550.062.50000420-21
ACCGACA7100.062.50000432-33
AGTACCC1550.062.50000418-19
TCACCAG1550.062.50000420-21
TTCAGTA1700.062.50000416-17
TTGTCTT150.003475553462.526-27
TGGTTCG150.003475553462.512-13
Produced by FastQC (version 0.11.5)