FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_QSER1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_QSER1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15467
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG942460.92972134221245No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG504132.59197000064653No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC860.5560224995150966No Hit
AGAAAGTGTTGTTGGTTCAGCTACCCTTGATCTTAAGAAGATCGGAAGAG530.3426650287709317No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT460.2974073834615633No Hit
GAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT390.252149738152195No Hit
AAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAGT310.20042671494148834No Hit
GAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT250.16163444753345835No Hit
AGAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT240.15516906963212002No Hit
AGAAAGTGTTGTTGGTTCAGTACCCTTGATCTTAAGAAGATCGGAAGAGC230.14870369173078166No Hit
AGAAAGTGTTGTTGGTTCAATGACACAACTTAACCAACAAATTGGCCAAG190.12284218012542833No Hit
AAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAGT180.11637680222409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTG152.1795217E-4125.01
AGAAAGT14950.0125.01
GAAAGTG15050.0124.169432
TGTTGTT15200.0122.9440847
GTGTTGT15200.0122.9440846
AGTGTTG15200.0122.9440845
AAGTGTT15200.0122.9440844
AAAGTGT15200.0122.9440843
TTGTTGG15200.0122.53299
GTTGTTG15200.0122.53298
CGCGAAT800.062.500004116-117
CGACAAA4650.062.50000434-35
ATCTCGG201.3681554E-462.500004122-123
AAGAGAT201.3681554E-462.50000476-77
TAAGAGA201.3681554E-462.50000476-77
ACCAACA10500.062.50000432-33
TCGGAAA201.3681554E-462.50000484-85
GAAGACG451.4551915E-1162.50000478-79
ACCGACA4650.062.50000432-33
ACACACG201.3681554E-462.50000490-91
Produced by FastQC (version 0.11.5)