FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_PIK3C2_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_PIK3C2_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23608
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT1524464.57133175194849No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT726830.786174178244664No Hit
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC590.24991528295493054No Hit
TCTTCCTTCTAATGATGAGCCCACGTCTGAACTCCAGTCACTGGTCGAAT550.23297187394103694TruSeq Adapter, Index 4 (96% over 27bp)
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC460.19484920365977634No Hit
TCTTCCTTTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC340.14401897661809557No Hit
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACTC260.11013215859030838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTTCT204.3682103E-6125.000013
TCTTCTA204.3682103E-6125.000014
CTTCTTC204.3682103E-6125.000012
TCTTCTT258.727693E-8125.01
TCTTCCT22950.0125.01
CTTCCTT22950.0125.02
TTCCTTC23000.0124.1847843
TCCTTCT23050.0123.915414
CCTTCTA23000.0123.913045
CTTCTAA23350.0122.5909966
TTCTAAT23300.0122.585837
TCTAATG23250.0122.580658
CTAATGA23250.0122.580659
TTCAAAA201.3756494E-462.500004120-121
CATTCAA201.3756494E-462.500004118-119
TCAAAAT201.3756494E-462.500004122-123
AAAATCT201.3756494E-462.500004124-125
ATTCAAA201.3756494E-462.500004120-121
CAAAATC201.3756494E-462.500004122-123
AGCCCAC255.484564E-662.518-19
Produced by FastQC (version 0.11.5)