FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_PIK3C2_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_PIK3C2_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23753
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT1538264.75813581442344No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT722730.42563044668042No Hit
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC560.23575969351239842No Hit
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC480.20207973729634154No Hit
TCTTCCTTCTAATGATGAGCACACGTCTGAACTCCAGTCACGGTCGAATC240.10103986864817077TruSeq Adapter, Index 9 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCT23450.0125.000011
CTTCCTT23450.0125.000012
TTCCTTT152.1942657E-4125.03
TTCCTTC23400.0124.465813
CCTTCTA23400.0124.198725
TCCTTCT23500.0123.936174
TTCTAAT23550.0123.673047
CTTCTAA23550.0123.673046
TCTAATG23500.0123.670218
CTAATGA23500.0123.670219
AGAATCT403.583409E-1062.500004124-125
GAACTCA201.3757392E-462.500004106-107
TCAGAAT451.4551915E-1162.500004122-123
CTAAAAC16150.062.50000438-39
GAGAGCA201.3757392E-462.50000492-93
CAGAATC403.583409E-1062.500004122-123
AATGATG23600.062.50000410-11
AACATAC16150.062.50000442-43
CGGAGAG150.003466040162.590-91
TGACTCC150.003466040162.5106-107
Produced by FastQC (version 0.11.5)