FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_PIK3C2_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_PIK3C2_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24197
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT1545263.85915609373063No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT777332.1238170021077No Hit
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC710.2934248047278588No Hit
TCTTACTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT530.21903541761375375No Hit
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC360.1487787742282101No Hit
TCTTACTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT300.12398231185684176No Hit
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACTC260.10745133694259619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCT24000.0125.01
CTTCCTT24000.0125.02
TCCTTCT24050.0124.740114
TTCCTTC24050.0124.740113
CCTTCTA24000.0124.7395865
TCTAATG24050.0124.480248
TTCTAAT24050.0124.480247
CTTCTAA24050.0124.480246
CTAATGA24100.0124.221999
CAGAATC451.4551915E-1162.500004122-123
CGCTGAA150.00346639562.5102-103
ACACGCT150.00346639562.598-99
ACGCTGA150.00346639562.5100-101
CACACGC150.00346639562.598-99
CACGCTG150.00346639562.5100-101
AATCAGG150.00346639562.570-71
GAATCAG150.00346639562.568-69
GCTGAAC150.00346639562.5102-103
TTTAGAC24000.062.36979352-53
TAGACAA24000.062.36979354-55
Produced by FastQC (version 0.11.5)