FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_PIK3C2_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_PIK3C2_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26359
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT1709564.85450889639213No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT797430.251526992678023No Hit
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC650.24659509086080658No Hit
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC540.20486361394590086No Hit
TCTTCCTTCTAATGATGAGCCCACGTCTGAACTCCAGTCACGGTCGAATC440.1669259076596229TruSeq Adapter, Index 11 (96% over 27bp)
TCTTCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACTC430.1631321370309951No Hit
TCTTCCTTCTAATGATGAGCACACGTCTGAACTCCAGTCACGGTCGAATC390.14795705451648394TruSeq Adapter, Index 9 (96% over 29bp)
TTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACTC310.11760688948746159No Hit
TCTTCCTTTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTC280.1062255776015782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCT25950.0125.01
CTTCCTT26050.0124.7600862
TCCTTCT26100.0124.281614
TTCCTTC26100.0124.281613
CCTTCTA26100.0124.281615
TCTAATG26200.0124.045798
CTAATGA26200.0124.045799
TTCTAAT26200.0124.045797
CTTCTAA26200.0124.045796
AGAATCT451.4551915E-1162.500004124-125
GAATCTC451.4551915E-1162.500004124-125
CAGAATC451.4551915E-1162.500004122-123
AGCCCAC150.00346815162.518-19
CGCTGAA150.00346815162.5102-103
CGTCACA150.00346815162.5112-113
ACACGCT150.00346815162.598-99
ACGCTGA150.00346815162.5100-101
CACACGC150.00346815162.598-99
GCCCACG150.00346815162.518-19
CACGCTG150.00346815162.5100-101
Produced by FastQC (version 0.11.5)