FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_NSFL1C_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_NSFL1C_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7598
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG407553.63253487759937No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG195525.730455382995526No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCT76710.094761779415636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATTGTCG1922.526980784416952No Hit
CCAACAGGCAGAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA160.21058173203474598No Hit
CCAACAGGCAGAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCTG130.17109765727823112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAGAGCACACGTCTG110.14477494077388786No Hit
CCAACAGGCAGAAAATGAAGAGCACACGTCTGAACTCCAGTCACTCCAAA100.13161358252171623TruSeq Adapter, Index 6 (96% over 33bp)
CCAACAGGCAGAAAATTCGGCTTGCAGACAGACGGAAGAGCACACGTCTG90.11845222426954462No Hit
CCAACAGGCAGAAAATGAAGCTTCCGATCTGTCTGCAAGCCAAATTCGGC80.10529086601737299No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAAGCACACGTCTG80.10529086601737299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAG7300.0125.01
GCAGAAA7500.0124.1666648
AGGCAGA7500.0124.1666646
CAGAAAA7500.0124.1666649
GGCAGAA7500.0124.1666647
CAACAGG7400.0124.15542
AACAGGC7400.0124.15543
CAGGCAG7400.0124.15545
ACAGGCA7400.0124.15544
AGCCTAC5600.062.50000456-57
TTGTCGA358.3164196E-962.50000444-45
ACAAACA6150.062.50000464-65
AGCTCTT6150.062.50000428-29
AGCCAGC6150.062.50000424-25
CCAGCTC6150.062.50000426-27
ATCCAAA6150.062.50000470-71
AAACATC6150.062.50000466-67
AACGAAT4150.062.50000446-47
CCAAAGC6150.062.50000420-21
CATCTTA6150.062.50000436-37
Produced by FastQC (version 0.11.5)