FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_NSFL1C_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_NSFL1C_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066
Sequences flagged as poor quality0
Sequence length131
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG413258.47721483158789No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG200128.318709312199264No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATTGTCG4916.948768751769034No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCT1752.476648740447212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACAGGCAGAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA100.1415227851684121No Hit
CCAACAGGCAGAAAATGAAACCAAAGCCAGCTCTTCCATCTTAATCAACG100.1415227851684121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAG7050.0125.01
CAACAGG7050.0125.02
GCAGAAA7000.0125.08
AGGCAGA7000.0125.06
AACAGGC7000.0125.03
CAGGCAG7000.0125.05
CAGAAAA7000.0125.09
ACAGGCA7000.0125.04
GGCAGAA7000.0125.07
AGCCTAC6100.062.50000456-57
GCCTACC6100.062.50000458-59
GAGCCTA6100.062.50000456-57
CAGAGCC6100.062.50000454-55
AGAGCCT6100.062.50000454-55
TCAGAGC6100.062.50000452-53
CAGCTCT6900.062.528-29
ACAAACA6900.062.564-65
AATCCAG150.003415594662.5114-115
ATCCAGT150.003415594662.5114-115
TCGACGA2050.062.544-45
Produced by FastQC (version 0.11.5)