FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_NSFL1C_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_NSFL1C_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22920
Sequences flagged as poor quality0
Sequence length131
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG1254554.73385689354275No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG666929.096858638743456No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCT14216.199825479930192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATTGTCG8183.5689354275741714No Hit
CCAACAGGCAGAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA300.13089005235602094No Hit
CCAACAGGCAGAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACGA240.10471204188481677No Hit
CAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA240.10471204188481677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAG22650.0125.01
GCAGAAA22750.0124.725278
CAGAAAA22750.0124.725279
CAACAGG22700.0124.724672
AACAGGC22700.0124.724673
ACAGGCA22700.0124.724674
GGCAGAA22700.0124.724677
AGGCAGA22750.0124.450556
CAGGCAG22750.0124.450555
CCAGCTC20450.062.50000426-27
AACGAAT12300.062.50000446-47
AATCAAC12300.062.50000442-43
GAAGCCA20450.062.50000416-17
TCAACGA12300.062.50000444-45
CATCACC150.00346524662.500004118-119
GACAATC150.00346524662.50000488-89
TCCATCA150.00346524662.500004116-117
CAGACAA150.00346524662.50000486-87
TTAATCA12300.062.50000440-41
AGACAAT150.00346524662.50000488-89
Produced by FastQC (version 0.11.5)