FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_NSFL1C_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_NSFL1C_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19557
Sequences flagged as poor quality0
Sequence length131
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG1180860.37735849056604No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG407920.85698215472721No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATTGTCG13376.836426854834586No Hit
CCAACAGGCAGAAAATTCGGCTTGCAGACAGATCGGAAGAGCACACGTCT8824.509894155545329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACAGGCAGAAAATGAAGCACACGTCTGAACTCCAGTCACTCCAAATT480.24543641662831722TruSeq Adapter, Index 3 (96% over 26bp)
CCAACAGGCAGAAAATGAAGTCTTTCCCTACACGACGCTCTTCCAAATTC310.15851101907245488Illumina Single End PCR Primer 1 (96% over 26bp)
CCAACAGGCAGAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA260.13294472567367183No Hit
CAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACGA220.1124916909546454No Hit
CCAACAGGCAGAAAATGAAGCTCTTTCCCTACACGACGCTCTTCCAAATT200.10226517359513218Illumina Single End Sequencing Primer (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAG19150.0125.000011
AACAGGC19300.0125.03
CAACAGG19250.0124.999992
TCAACAG152.1883627E-4124.999991
CAGGCAG19300.0124.676165
ACAGGCA19300.0124.676164
GCAGAAA19350.0124.3540048
AGGCAGA19350.0124.3540046
GGCAGAA19350.0124.3540047
CAGAAAA19300.0124.3523259
AGCCTAC15700.062.50000456-57
TTGTCGA1300.062.50000444-45
TCCTACC850.062.50000458-59
TCGAATC1300.062.50000448-49
ATGAAGC18000.062.50000414-15
GTCGAAT1300.062.50000446-47
ATCAGAT950.062.50000452-53
AGAGCCT15700.062.50000454-55
GATCCTA850.062.50000456-57
ATTGTCG1300.062.50000444-45
Produced by FastQC (version 0.11.5)