FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_IMMT_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_IMMT_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30675
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC2960096.49551752241238No Hit
CAGTCGGGTCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT480.15647921760391198No Hit
CAGTCGGGTCCCTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT420.136919315403423No Hit
CAGTCGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT410.1336593317033415No Hit
CAGTCGGGTTCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC370.12061939690301549No Hit
CAGTCGGGTCCATAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT370.12061939690301549No Hit
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAACAAAAGAATT360.117359413202934No Hit
TAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC320.10431947840260798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG30400.0125.000011
GGTCCAT152.2004619E-4125.07
AGTCGGG30500.0124.795082
GTCGGGT30500.0124.795083
TCGGGTC30500.0124.795084
CGGGTCC30450.0124.794745
GGGTCCA30350.0124.794076
GGTCCAC30200.0124.793057
TCCACTA30200.0124.58619
GTCCACT30150.0124.585418
AACAGCC19850.062.50000452-53
GCCTGCC19850.062.50000456-57
CAGCCTG19850.062.50000454-55
GGAGAGC600.062.5114-115
ACACGCT150.003470857262.5122-123
ACGCTGA150.003470857262.5124-125
CGGAGAG600.062.5114-115
CAGCAAA150.003470857262.5100-101
TCGGAGA600.062.5112-113
GAAGACA302.2089444E-762.5116-117
Produced by FastQC (version 0.11.5)