FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_IMMT_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_IMMT_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC966695.7029702970297No Hit
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAACAAAAGAATT780.7722772277227723No Hit
CAGTCGGGTCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT190.18811881188118812No Hit
CAGTCGGGTCCATTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC140.1386138613861386No Hit
CAGTCGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT110.10891089108910892No Hit
CAGTAGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC110.10891089108910892No Hit
CAGTCGGGTCCATAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT110.10891089108910892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG9750.0125.000011
AGTCGGG9650.0125.000012
GGGTCCA9800.0123.7244956
CGGGTCC9750.0123.717965
GTCGGGT9750.0123.717963
TCGGGTC9750.0123.717964
TCCACTA9950.0122.487449
GGTCCAC9900.0122.474757
GTCCACT9900.0122.474758
GGAGATA10050.062.50000484-85
ATCTCTA10050.062.50000418-19
AATCTAA9950.062.50000446-47
GCCTCAC10050.062.50000460-61
TAAACAG10050.062.50000450-51
AAATCTC10050.062.50000416-17
AGAAGTA9950.062.50000432-33
CAACTCC10050.062.50000466-67
CACAACT10050.062.50000464-65
GTCTGCC3600.062.50000456-57
ACTCCAA10050.062.50000468-69
Produced by FastQC (version 0.11.5)