FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_IMMT_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_IMMT_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26541
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC2272385.61470931765946No Hit
CAGTCGGGTCCACTAAAAATCTTTCCCTCCAGCTTCAGCAACAGAGATCG1710.6442861987114276No Hit
CAGTCGGGTCCACTAAAAATCCAGCTTCAGCAACAGAGATCGGAAGAGCA1070.4031498436381447No Hit
CAGTCGGGTCCACTAAAAATCTTCCGATCTCCAGCTTCAGCAACAGAGAT1030.3880788214460646No Hit
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAACAAAAGAATT930.35040126596586413No Hit
CAGTCGGGTCCACTAAAAATCTCTTTCCCTCCAGCTTCAGCAACAGAGAT840.3164914660336837No Hit
CAGTCGGGTCCACTAAAAATCTTTCCCTACACGACGCTCTTCCAGCTTCA810.3051881993896236Illumina Single End PCR Primer 1 (95% over 24bp)
CAGTCGGGTCCACTAAAAATCCGATCTCCAGCTTCAGCAACAGAGATCGG790.29765268829358354No Hit
CAGTCGGGTCCACTAAAAATCTCTTCCGATCTCCAGCTTCAGCAACAGAG720.2712783994574432No Hit
CAGTAGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC650.24490411062130288No Hit
CAGTCGGGTCCACTAAAAATCTTTCCCTCCACTTCAGCAACAGAGATCGG560.2109943106891225No Hit
CAGTCGGGTCCACTAAAAATCGTGTGCTCTTCCGATCTCCAGCTTCAGCA480.18085226630496215No Hit
CAGTCGGGTCCACTAAAAATCTCTTTCCCTACACGACGCTCTTCCAGCTT460.17331675520892204Illumina Single End PCR Primer 1 (96% over 26bp)
CAGTCGGGTCCACTAAAAATTCCGATCTCCAGCTTCAGCAACAGAGATCG420.15824573301684186No Hit
CAGTCGGGTCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT360.1356391997287216No Hit
CAGTCGGGTCCACTAAAAATCCTCTTCCGATCTCCAGCTTCAGCAACAGA350.13187144418070157No Hit
CAGTCGGGTCCATAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT350.13187144418070157No Hit
CAGTCGGGTCCACTAAAAATCTCTTTCCCTCCACTTCAGCAACAGAGATC340.1281036886326815No Hit
CAGTCGGGTCCACTAAAAATCTCTCCAGCTTCAGCAACAGAGATCGGAAG330.12433593308466147No Hit
CAGTCGGGTCCACTAAAAATCTTTCCAGCTTCAGCAACAGAGATCGGAAG320.12056817753664142No Hit
CAGTCGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT310.1168004219886214No Hit
CAGTCGGGTCCACTAAAAATTCTTTCCCTCCAGCTTCAGCAACAGAGATC310.1168004219886214No Hit
CAGTCGGGTCCACTAAAAATCCGTGTGCTCTTCCGATCTCCAGCTTCAGC310.1168004219886214No Hit
CAGTCGGGTCCACTAAAAATCTCCAGCTTCAGCAACAGAGATCGGAAGAG290.10926491089258128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG26300.0124.999991
AGTCGGG26350.0124.762822
GTCGGGT26300.0124.762353
TCGGGTC26300.0124.762354
CGGGTCC26100.0124.760545
GGGTCCA26200.0124.2843556
GGTCCAC26150.0124.282987
TCCACTA26050.0124.280229
GTCCACT26050.0124.280228
ATCTCTT600.062.50000418-19
AATCTCC150.003468259562.50000418-19
ATCCAGC150.003468259562.50000418-19
CAGCAAA150.003468259562.500004100-101
AAATTCA150.003468259562.500004118-119
TCGAAGA150.003468259562.500004112-113
AAGAGAT150.003468259562.500004106-107
GATCGAA150.003468259562.500004110-111
CGAAGAG150.003468259562.500004114-115
GAAGACA150.003468259562.500004116-117
ACACACG150.003468259562.500004120-121
Produced by FastQC (version 0.11.5)