FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_IMMT_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_IMMT_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19123
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC1800894.16932489672124No Hit
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAACAAAAGAATT2831.4798933221774826No Hit
CAGTCGGGTCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT360.18825498091303666No Hit
CAGTCGGGTCCATAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT270.14119123568477748No Hit
CAGTCGGGTCTACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC240.1255033206086911No Hit
CAGTCGGGTCCCTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT230.12027401558332898No Hit
CAGTCGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT230.12027401558332898No Hit
TAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC210.10981540553260472No Hit
AGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATCT210.10981540553260472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG18800.0125.000011
AGTAGGG152.1875766E-4125.02
AGGGTCC152.1875766E-4125.05
GTAGGGT152.1875766E-4125.03
TAGGGTC152.1875766E-4125.04
AGTCGGG18850.0124.336872
GTCGGGT18850.0124.336873
TCGGGTC18800.0124.335114
CGGGTCC18700.0124.331545
GGGTCCA18850.0124.00536
GGTCCAC18800.0124.002667
GTCCACT18800.0124.002668
TCCACTA18900.0123.677249
AACGTCT150.003460835262.5122-123
AAGTAAC302.1867163E-762.534-35
AATTTAA302.1867163E-762.546-47
GAAGACA150.003460835262.5116-117
AGAATTT302.1867163E-762.544-45
GCAACGT150.003460835262.5120-121
ACACACG150.003460835262.5120-121
Produced by FastQC (version 0.11.5)