FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_HEATR1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_HEATR1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10543
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC639560.65635966992318No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGC338932.14455088684435No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGATC860.8157071042397799No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAATC480.4552783837617377No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGGATC370.35094375414967277No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC220.20866925922412974No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGCAAAGAGAGAGCAGGAGC180.17072939391065162No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGAGC170.16124442758228208No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGAATC170.16124442758228208No Hit
ATCTTCCTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGCC150.14227449492554303No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAAGC150.14227449492554303No Hit
ATCTTCCTTTTTTATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC140.13278952859717347No Hit
ATCTTCCTTTTTTATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC120.11381959594043442No Hit
ATCTTTCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC120.11381959594043442No Hit
ATTTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC120.11381959594043442No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAATC110.10433462961206487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT10400.0125.05
ATCTTCC10400.0125.01
TTTTTGA10300.0125.08
TTCCTTT10400.0125.04
TCTTCCT10400.0125.02
CTTCCTT10400.0125.03
CTTTTTG10300.0125.07
CCTTTTT10400.0124.399036
TTTTGAT10350.0124.396149
TCCTTGA201.35797E-462.50000448-49
ACGTCTG10350.062.500004118-119
AGAAGAT10350.062.500004100-101
GATACCC10350.062.50000412-13
AAACAGG10450.062.50000480-81
TAAGTAA10450.062.50000428-29
GCAGGAG6500.062.50000442-43
TATAAGT10450.062.50000426-27
GAAAACA10450.062.50000478-79
GTCTGAA10350.062.500004120-121
CCTCTGG10450.062.50000460-61
Produced by FastQC (version 0.11.5)