FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_HEATR1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_HEATR1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7457
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC439458.92450046935765No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGC248933.378034061955205No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGATC660.8850744267131554No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAATC370.4961780877028295No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGGATC230.3084350274909481No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGAGC220.2950248089043851No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGAATC180.2413839345581333No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC160.2145634973850074No Hit
ATCTTCCTTTTTTATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC120.1609226230387555No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGCAAAGAGAGAGCAGAAGC100.1341021858656296No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAATC90.12069196727906666No Hit
ATCTTCCTTTTTGATACCTAGCATTTTATAAGTAAAGAGAGAGCAGGAGC90.12069196727906666No Hit
ATCTTCCTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGCC80.1072817486925037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTCC7400.0125.01
TCCTTTT7450.0124.161075
TTCCTTT7450.0124.161074
CCTTTTT7450.0124.161076
TCTTCCT7450.0124.161072
CTTCCTT7450.0124.161073
CTTTTTG7450.0124.161077
TTTTGAT7400.0124.15549
TTTTTGA7400.0124.15548
ACACGTC7350.062.500008116-117
CAGCATT7350.062.50000818-19
ACGTCTG7350.062.500008118-119
AAGAGCA7350.062.500008110-111
GAGCACA7350.062.500008112-113
GGAAGAG7350.062.500008108-109
TCCTCTG201.3450309E-462.50000460-61
TCCTTGA201.3450309E-462.50000448-49
ATGATTC201.3450309E-462.50000454-55
AGAAGAA201.3450309E-462.50000496-97
TGATTCC201.3450309E-462.50000456-57
Produced by FastQC (version 0.11.5)