FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n01_ms_HEATR1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n01_ms_HEATR1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC587949.482366804141066No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAAGC512243.1108492551132No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGATC720.6060095951519232No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGAATC700.589175995286592No Hit
ATCTTCCTTTTTGATACCCAGCTTGGGGGAGAAGAGAAGATCGGAAGAGC380.31983839744129283No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAATC270.22725359818197124No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGGATC230.19358639845130882No Hit
ATCTTCCTTTTTGATACCCCGCATTTTATAAGTAAAGAGAGATCAGAATC210.17675279858597762No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGCAAAGAGAGAGCAGAAGC190.15991919872064642No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGCAAAGAGAGAGCAGGAGC170.14308559885531522No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGATC160.1346687989226496No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGGAGC140.1178351990573184No Hit
ATCTTCCTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGCC120.10100159919198722No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGATCAGAAGC120.10100159919198722No Hit
ATTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGCC120.10100159919198722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT11800.0125.05
ATCTTCC11800.0125.01
TTCCTTT11800.0125.04
CCTTTTT11800.0125.06
TCTTCCT11800.0125.02
CTTCCTT11800.0125.03
TTTTGAT11850.0124.4725659
TTTTTGA11850.0124.4725658
CTTTTTG11850.0124.4725657
GAGCAGG6250.062.50000440-41
AGCAGGA6250.062.50000442-43
GCAGAAG5350.062.50000442-43
AAGCCTT5400.062.50000446-47
TGATGAT11700.062.552-53
TCCTCTG255.400647E-662.560-61
GCCTTGA11500.062.548-49
CAGCATT11800.062.518-19
CCCAGCA11800.062.516-17
ATGATTC255.400647E-662.554-55
ATGATGC11450.062.554-55
Produced by FastQC (version 0.11.5)